TCRab/Vb2 antibody (SPRD)

Catalog No. ABIN376730

The Mouse Monoclonal anti- antibody has been validated for FACS. It is suitable to detect in samples from Chicken.

Quick Overview for TCRab/Vb2 antibody (SPRD) (ABIN376730)

Target: TCRab/Vb2

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: SPRD

Application: Flow Cytometry (FACS)

Clone: TCR-3

Product Details

Brand: Spectral Red ™

Specificity: Chicken/Peacock/Guinea Fowl TCRαβ/Vβ2, Mr 48 & 40 kDa

Characteristics: Mouse Anti-Chicken TCRab/Vb2-SPRD

Purification: Purified

Immunogen: CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Depletion - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.5 g/106 cells SPRD conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In ovo suppression of the development of TCRab-Vb2 cell

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: TCRab/Vb2

Background: In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR2 and TCR3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct V β families, V β 1 and V β 2, respectively. The TCR3 MAb reacts with approximately 9 % of thymocytes, 15-25 % of blood mononuclear cells and 13 % of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR3 + thymocytes include CD4 + CD8 -, CD4 - CD8 +, CD4 + CD8 + and CD4 - CD8 - subpopulations. The TCR3 + thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4 + CD8 +. Cells in the other subset express TCR3/CD3 in higher density and are either CD4 + CD8 - or CD4 - CD8 +, corresponding to the more mature medullary subset of thymocytes. The TCR3 + cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are “single positive, with CD4 + CD8 - cells being four times more frequent that the CD4 - CD8 + cells (ca. 80 % CD4 + vs ca. 20 % CD8 + ).